9O6J

Structure of Siglec-10 in complex with 2,3-Sialyllactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-04
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97741
Spacegroup name	P 65 2 2
Unit cell lengths	81.765, 81.765, 485.480
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.550 - 3.390
R-factor	0.2462
Rwork	0.244
R-free	0.29190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.187
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.550	2.240
High resolution limit [Å]	2.170	2.170
Rmerge	0.597	3.349
Rmeas	0.615	3.447
Number of reflections	47203	1162
<I/σ(I)>	2.2
Completeness [%]	89.3
Redundancy	15.4	1.8
CC(1/2)	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.085 M Sodium Citrate pH 5.4, 0.17 M Ammonium Acetate, 15% v/v Glycerol, and 28% w/v PEG 4000