9O6I

TUBULIN-RB3_SLD IN COMPLEX WITH COMPOUND QW-5-70

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-03
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92012
Spacegroup name	P 21 21 21
Unit cell lengths	65.843, 127.095, 251.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.950 - 2.510
R-factor	0.2114
Rwork	0.210
R-free	0.24390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.592
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.960	2.570
High resolution limit [Å]	2.510	2.510
Number of reflections	73305	5229
<I/σ(I)>	10.8	1.8
Completeness [%]	99.8	97.7
Redundancy	8.3	7.6
CC(1/2)	0.997	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293.15	0.1 M trisodium citrate, pH 5.6, 0.2 M ammonium sulfate, 12.2% PEG 4000