9O3K

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolide erythromycin, mRNA, aminoacylated A-site Lys-tRNAlys, P-site fMAC-peptidyl-tRNAmet, and deacylated E-site tRNAlys at 2.70A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-24
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	209.690, 447.930, 622.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	152.210 - 2.700
R-factor	0.2193
Rwork	0.217
R-free	0.26210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8cvk
RMSD bond length	0.004
RMSD bond angle	0.810
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	152.210	152.210	2.770
High resolution limit [Å]	2.700	12.070	2.700
Rmerge	0.160	0.068	1.392
Rmeas	0.190	0.081	1.647
Total number of observations	5456260
Number of reflections	1574939	18436	116275
<I/σ(I)>	6.16	16.67	0.99
Completeness [%]	99.5	98.5	99.8
Redundancy	3.464	3.498	3.504
CC(1/2)	0.988	0.992	0.165

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME