9O0D

crystal structure of rSAMD9 tRNase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-08
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.97918
Spacegroup name	P 21 21 2
Unit cell lengths	107.984, 142.486, 83.133
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.476 - 2.391
R-factor	0.2302
Rwork	0.228
R-free	0.27710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.394
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.391	2.391
Rmerge	0.081	1.138
Number of reflections	51039	4618
<I/σ(I)>	13.4
Completeness [%]	99.8	93.7
Redundancy	6.3
CC(1/2)	0.994	0.738

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.2 M Magnesium formate dihydrate, 20% w/v Polyethylene glycol 3,350, 0.1M Hepes, pH 7.0.