9O0B

X-ray Crystal Structure of Fission Yeast Fsc1 protein in P43212

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	58.003, 58.003, 488.271
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.010 - 2.504
Rwork	0.244
R-free	0.29830
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.050	2.681
High resolution limit [Å]	2.500	2.504
Number of reflections	32222	5436
<I/σ(I)>	8.4
Completeness [%]	99.3
Redundancy	4
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG3350, 0.2M magnesium chloride, 0.1M Tris, pH 8.5