9O00

Crystal structure of AfOgg1 in a DNA-free state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2000-05-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	F 2 2 2
Unit cell lengths	86.508, 106.499, 141.698
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.570 - 1.930
R-factor	0.2178
Rwork	0.215
R-free	0.24260
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.235
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.570	1.980
High resolution limit [Å]	1.930	1.930
Rmerge	0.075	1.374
Number of reflections	21581	906
<I/σ(I)>	16.3
Completeness [%]	87.7
Redundancy	6
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	100 mM imidazole/malate, pH 5.0, 75% ammonium sulfate (saturated)