9NZK
Crystal structure of Yck2 with LY364947
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-05 |
| Detector | DECTRIS EIGER R 1M |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 91.532, 91.532, 118.824 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.950 - 1.700 |
| R-factor | 0.1761 |
| Rwork | 0.174 |
| R-free | 0.21110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.112 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.950 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.622 |
| Number of reflections | 40815 | 4046 |
| <I/σ(I)> | 19.25 | 0.48 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 3.4 | 1.7 |
| CC(1/2) | 0.999 | 0.445 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1 M Tris pH 8, 25 mM Magnesium chloride, 20% (w/v) PEG3350 |
