9NZ8

Crystal structure of human OGG1 in a DNA-free state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	P 65
Unit cell lengths	106.731, 106.731, 47.817
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.610 - 1.850
R-factor	0.1717
Rwork	0.170
R-free	0.19860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.798
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.610	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.111
Number of reflections	26819	1644
<I/σ(I)>	12
Completeness [%]	99.8	97.1
Redundancy	9.7
CC(1/2)	0.998	0.240

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	288	100 mM sodium citrate, pH 5.0, 22% PEG6000, 5% ammonium sulfate (saturated)