9NZ1

Adeno-associated virus serotype 11 basic regions in complex with importin alpha 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.525, 90.634, 98.992
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.130 - 2.680
R-factor	0.2236
Rwork	0.223
R-free	0.23910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.449
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.130	2.810
High resolution limit [Å]	2.680	2.680
Rmerge	0.074	0.403
Rmeas	0.084	0.460
Rpim	0.040	0.216
Number of reflections	80640	2657
<I/σ(I)>	8.4	2
Completeness [%]	98.6	99.8
Redundancy	4	4.1
CC(1/2)	0.996	0.868

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	0.65 M Na citrate, 0.01 M DTT, 0.1 M HEPES pH 7.0