9NY9
Crystal structure of the post-reactive state of porcine OAS1 in complex with dsRNA, catalytic center bound PPi, and dissociated 25A2.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.405, 71.405, 203.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.010 - 2.740 |
| Rwork | 0.234 |
| R-free | 0.25360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.574 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.010 | 2.840 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.166 | 0.739 |
| Number of reflections | 14649 | 1448 |
| <I/σ(I)> | 9.05 | |
| Completeness [%] | 99.8 | |
| Redundancy | 13.36 | |
| CC(1/2) | 0.998 | 0.946 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 291 | 50 mM Tris HCl pH 8.3, 100 mM KCl, 10 mM MgCl2, 23 % (v / v) PEG400 |
