9NXM

Crystal structure of Arabidopsis thaliana NUDT11 E90Q/E94Q mutant

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	82.572, 82.572, 86.847
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.290 - 2.410
R-factor	0.215
Rwork	0.211
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.908
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.290	41.290	2.550
High resolution limit [Å]	2.400	7.120	2.410
Rmeas	0.092	0.029	0.888
Number of reflections	25631	993	4108
<I/σ(I)>	16.34		2.09
Completeness [%]	99.6		98.8
Redundancy	6.78
CC(1/2)	0.999	0.999	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	295	17% PEG3350, 100mM phosphate-citrate pH4.0, 0.2M NaCl. Drop was set up with microseeding.