9NXJ

The GH43 domain of an alpha-l-arabinofuranosidase (AtAbf43C_GH43) from Acetivibrio thermocellus DSM1313

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92015
Spacegroup name	P 65
Unit cell lengths	105.304, 105.304, 128.856
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.590 - 2.320
R-factor	0.2049
Rwork	0.203
R-free	0.24690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.992
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.590	2.380
High resolution limit [Å]	2.320	2.320
Rmerge	0.118	0.852
Rmeas	0.121	0.874
Rpim	0.027	0.192
Total number of observations	724982	50475
Number of reflections	35192	2503
<I/σ(I)>	21.2	3.3
Completeness [%]	99.7
Redundancy	20.6	20.2
CC(1/2)	0.999	0.867

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	PEGmme 5000, pH 5.5 Bis-Tris, AmS04