9NX3
Crystal structure of GP232 tail fiber recognition domain from mycobacteriophage Bxz-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2012-11-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.838, 88.387, 96.326 |
| Unit cell angles | 96.16, 102.08, 107.98 |
Refinement procedure
| Resolution | 41.310 - 1.900 |
| R-factor | 0.1841 |
| Rwork | 0.182 |
| R-free | 0.22020 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.812 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.20.1_4487) |
| Refinement software | REFMAC (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.052 | 0.413 |
| Number of reflections | 126628 | 4081 |
| <I/σ(I)> | 22 | |
| Completeness [%] | 93.9 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9 | 293 | 100 mM bicine pH 9.0, 20% PEG200, 4 mM beta-octyl-glucoside, supplemented with trehalose |
