9NWU
Crystal structure of a high affinity VL-VH tetrabody for the erythropoietin receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-04 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.355, 141.779, 60.651 |
| Unit cell angles | 90.00, 90.76, 90.00 |
Refinement procedure
| Resolution | 70.890 - 1.960 |
| R-factor | 0.1691 |
| Rwork | 0.167 |
| R-free | 0.21520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.978 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 141.780 | 2.010 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.050 | 0.716 |
| Rmeas | 0.060 | 0.947 |
| Rpim | 0.032 | 0.610 |
| Number of reflections | 65686 | 3298 |
| <I/σ(I)> | 14.9 | 1.3 |
| Completeness [%] | 94.9 | 71.3 |
| Redundancy | 3.3 | 2.3 |
| CC(1/2) | 0.998 | 0.444 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 295 | 20% PEG8000, 100 mM CHES pH 9.5, with microseeding. Crystals cryoprotected with the same crystallization liquor plus 25% ethylene glycol |
