9NWN

Structure of minimal CRISPR polymerase bound to ApNHpp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 2
Unit cell lengths	49.135, 56.769, 93.705
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.550 - 2.280
R-factor	0.2269
Rwork	0.224
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.958
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.550	2.370
High resolution limit [Å]	2.280	2.280
Rmerge	0.146
Rmeas	0.159
Rpim	0.061
Number of reflections	12367	1349
<I/σ(I)>	6.8
Completeness [%]	98.8	100
Redundancy	6.6
CC(1/2)	0.998	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1 M Bicine pH 8.0, 15% PEG 1500