9NW9
CA117v2v8 Fab bound to HLA-E-RL9HIV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2022-11-03 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.540, 72.520, 122.350 |
| Unit cell angles | 90.00, 100.79, 90.00 |
Refinement procedure
| Resolution | 46.270 - 2.300 |
| R-factor | 0.1935 |
| Rwork | 0.191 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.944 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_3758) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.520 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 43168 | 4132 |
| <I/σ(I)> | 13.9 | 2 |
| Completeness [%] | 98.0 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.998 | 0.757 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M magnesium chloride, 20% PEG 3350 |
