9NW8

CA117v2v8 Fab bound to HLA-E-Mtb44

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-10-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	57.730, 72.330, 121.270
Unit cell angles	90.00, 101.36, 90.00

Refinement procedure

Resolution	59.450 - 2.300
R-factor	0.1983
Rwork	0.196
R-free	0.24570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.891
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_3758)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.590	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	42884	4178
<I/σ(I)>	5.7	0.7
Completeness [%]	98.6
Redundancy	3.4
CC(1/2)	0.989	0.312

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M magnesium acetate, 20% PEG 3350