9NW6

CA147v24 Fab bound to HLA-E-VL9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-06-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	73.680, 92.860, 319.259
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.810 - 3.100
R-factor	0.2401
Rwork	0.239
R-free	0.25830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.295
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_3758)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.860	3.230
High resolution limit [Å]	3.100	3.100
Number of reflections	40717	4528
<I/σ(I)>	5.1	1.7
Completeness [%]	99.7
Redundancy	6.1
CC(1/2)	0.589	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M potassium iodide, 0.1 M MES pH 6.5, 25% PEG 4000