9NW4

Structure of CISV1 antibody bound to PvCSP repeat peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-06
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97741
Spacegroup name	P 1 21 1
Unit cell lengths	51.498, 66.958, 142.806
Unit cell angles	90.00, 96.19, 90.00

Refinement procedure

Resolution	48.760 - 1.820
R-factor	0.18431
Rwork	0.183
R-free	0.21520
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PDB-REDO

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.760	1.850
High resolution limit [Å]	1.820	1.820
Rmerge	0.070	0.566
Rmeas	0.084	0.671
Rpim	0.045	0.358
Total number of observations		15576
Number of reflections	85963	4551
<I/σ(I)>	10	2.2
Completeness [%]	99.2
Redundancy	3.4	3.4
CC(1/2)	0.998	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	300	0.1M Imidazole, pH 8.0, 1M Na3Citrate