9NU9

X-ray Crystal Structure of Fission Yeast Fsc1 protein in C2 Symmetry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	347.817, 40.912, 59.095
Unit cell angles	90.00, 92.57, 90.00

Refinement procedure

Resolution	47.840 - 2.450
R-factor	0.2066
Rwork	0.204
R-free	0.24960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.971
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.840	2.540
High resolution limit [Å]	2.450	2.500
Number of reflections	29304	1340
<I/σ(I)>	8.3
Completeness [%]	93.8
Redundancy	3.2
CC(1/2)	0.989	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 3350, magnesium chloride, Tris