9NTN

Structure of Cap10-CdnD complex containing NDG modification

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	70.459, 82.920, 231.779
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.870 - 2.430
R-factor	0.2373
Rwork	0.235
R-free	0.27770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.786
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.870	2.470
High resolution limit [Å]	2.430	2.430
Rmerge	0.381	3.675
Rmeas	0.396	3.814
Rpim	0.106	1.017
Number of reflections	52191	2671
<I/σ(I)>	5.28	0.36
Completeness [%]	99.3	94.33
Redundancy	13.7	13.9
CC(1/2)	0.993	0.348

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291.15	0.1 M HEPES-KOH pH 7.5, 0.3 M calcium acetate, 10% PEG-8000