9NTH
Helix pomatia AMP deaminase (HPAMPD) in complex with Pentostatin monophosphate (PMP)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-07 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.992, 81.936, 212.195 |
| Unit cell angles | 90.00, 92.13, 90.00 |
Refinement procedure
| Resolution | 42.730 - 1.610 |
| R-factor | 0.1681 |
| Rwork | 0.167 |
| R-free | 0.19870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.321 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.730 | 1.640 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rpim | 0.084 | 0.647 |
| Number of reflections | 332916 | 16484 |
| <I/σ(I)> | 5.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.995 | 0.334 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M BIS-TRIS pH 6.5, 20% w/v Polyethylene glycol monomethyl ether 5,000 |
