9NT6
Crystal structure of ecLacI transcription factor ancestor 4 bound to Beta-methyl-D-galactoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.467, 108.336, 119.601 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.340 - 1.500 |
| R-factor | 0.1772 |
| Rwork | 0.176 |
| R-free | 0.20780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.988 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.340 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.140 | 1.767 |
| Rmeas | 0.146 | 1.853 |
| Rpim | 0.041 | 0.552 |
| Total number of observations | 986424 | 40988 |
| Number of reflections | 76948 | 3714 |
| <I/σ(I)> | 10.8 | 1.4 |
| Completeness [%] | 99.9 | |
| Redundancy | 12.8 | 11 |
| CC(1/2) | 0.998 | 0.653 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 21 % PEG 3350, 0.1M HEPES pH 7.1 |
