9NT5
Crystal structure of ecLacI transcription factor ancestor 1 bound to glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 104.821, 104.821, 50.126 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.310 - 1.670 |
| R-factor | 0.1899 |
| Rwork | 0.189 |
| R-free | 0.21210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.719 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.390 | 1.700 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.062 | 5.031 |
| Rmeas | 0.064 | 5.156 |
| Rpim | 0.014 | 1.122 |
| Total number of observations | 762570 | 39051 |
| Number of reflections | 37043 | 1862 |
| <I/σ(I)> | 22.5 | 0.7 |
| Completeness [%] | 100.0 | |
| Redundancy | 20.6 | 21 |
| CC(1/2) | 1.000 | 0.362 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 14.4% PEG 8000, 0.08 M sodium cacodylate pH 6.2, 0.16 M calcium acetate, 20 % glycerol |
