9NT4
Crystal structure of ecLacI transcription factor ancestor 1 bound to d-fucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.446, 210.997, 87.315 |
| Unit cell angles | 90.00, 90.51, 90.00 |
Refinement procedure
| Resolution | 43.660 - 2.400 |
| R-factor | 0.2212 |
| Rwork | 0.219 |
| R-free | 0.27010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.688 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.660 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.199 | 1.285 |
| Rmeas | 0.215 | 1.383 |
| Rpim | 0.080 | 0.509 |
| Total number of observations | 629149 | 33242 |
| Number of reflections | 88505 | 4558 |
| <I/σ(I)> | 8.4 | 1.6 |
| Completeness [%] | 99.1 | |
| Redundancy | 7.1 | 7.3 |
| CC(1/2) | 0.994 | 0.395 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 15%w/v PEG 4000, 0.2 M MgCl2, 0.1 M Tris-HCl, pH 8.5 |
