9NT1

Crystal structure of Lingulodinium polyedra MIF (lpMIF)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-19
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	98.308, 98.350, 98.598
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.220 - 1.500
R-factor	0.1996
Rwork	0.199
R-free	0.21550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.948
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.18_3845: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.220	1.554
High resolution limit [Å]	1.500	1.500
Rmerge	0.052
Number of reflections	76502	7588
<I/σ(I)>	27.5
Completeness [%]	100.0
Redundancy	13.1
CC(1/2)	0.999	0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	40 % PEG 400 MES ph 6.0, cryoprotected with 30% Ethylene glycol.