9NSD
Crystal structure of PvCSS-PvPTRAMP in complex with nanobody D7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953739 |
| Spacegroup name | P 32 |
| Unit cell lengths | 125.217, 125.217, 106.466 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.320 - 3.140 |
| R-factor | 0.2271 |
| Rwork | 0.225 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.668 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.320 | 3.252 |
| High resolution limit [Å] | 3.140 | 3.140 |
| Rpim | 0.046 | 0.497 |
| Number of reflections | 32565 | 3250 |
| <I/σ(I)> | 13.19 | |
| Completeness [%] | 99.9 | 99.88 |
| Redundancy | 5.4 | 5.4 |
| CC(1/2) | 0.544 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2M ammonium sulphate, 16% (w/v) PEG 3,350 |
