9NS6

KslB apoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-23
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.99
Spacegroup name	P 32
Unit cell lengths	95.593, 95.593, 193.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.800 - 2.950
R-factor	0.2159
Rwork	0.207
R-free	0.24780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.268
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.800	3.000
High resolution limit [Å]	2.950	2.950
Number of reflections	40480	2058
<I/σ(I)>	5.2
Completeness [%]	97.2
Redundancy	2.6
CC(1/2)	0.972	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	PEG 3350, Sodium Chloride, bisTRIS