9NRR
Crystal structure of the H5 influenza virus hemagglutinin from A/duck/France/161108h/2016 (H5N8) clade 2.3.4.4b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-31 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.376, 171.602, 226.001 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.560 - 1.940 |
| R-factor | 0.1676 |
| Rwork | 0.167 |
| R-free | 0.18780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.842 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21rc1_5127: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.560 | 1.970 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rpim | 0.050 | 0.600 |
| Number of reflections | 270800 | 13249 |
| <I/σ(I)> | 20.9 | 1 |
| Completeness [%] | 99.9 | |
| Redundancy | 9.8 | |
| CC(1/2) | 0.996 | 0.538 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris, pH 7.0, 0.11 M lithium sulfate and 2.0 M ammonium sulfate |
