9NRQ
GloR with glyoxal modification
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-01-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 100.482, 119.111, 34.225 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.400 - 2.090 |
| R-factor | 0.2222 |
| Rwork | 0.219 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.065 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.400 | 2.150 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.146 | 1.284 |
| Rmeas | 0.164 | 1.448 |
| Rpim | 0.053 | 0.655 |
| Number of reflections | 24888 | 1854 |
| <I/σ(I)> | 6.2 | 1.5 |
| Completeness [%] | 98.9 | 91.5 |
| Redundancy | 9.2 | 8.8 |
| CC(1/2) | 0.997 | 0.819 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M NaNO3, 0.1 M BisTris Propane pH 8.5, 22.5% PEG 3350 |
