9NR3

CRBN-DDB1 in complex with GLUL-cN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2024-04-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	76.435, 100.364, 169.672
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.270 - 2.930
R-factor	0.2519
Rwork	0.250
R-free	0.27840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.669
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.270	2.960
High resolution limit [Å]	2.930	2.930
Number of reflections	28693	923
<I/σ(I)>	7.8
Completeness [%]	99.7
Redundancy	6.5
CC(1/2)	0.995	0.363

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	8% v/v TacsimateTM pH 6.0, 20% w/v Polyethylene glycol 3,350