9NP7

Crystal Structure of Beat-Vb IG1+2 Domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	120
Detector technology	CCD
Collection date	2015-04-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03318
Spacegroup name	P 62 2 2
Unit cell lengths	183.680, 183.680, 68.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.920 - 3.400
R-factor	0.2975
Rwork	0.296
R-free	0.31500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.669
Data reduction software	XDS (20220220)
Data scaling software	XDS
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (dev_5533)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.490
High resolution limit [Å]	3.400	3.400
Rmerge	0.158	3.259
Rmeas	0.160	3.306
Number of reflections	9752	657
<I/σ(I)>	18.82	1.71
Completeness [%]	99.4	95.5
Redundancy	40.9	34.7
CC(1/2)	0.994	0.402

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	4.8% (w/v) PEG 3350, 22% (v/v) PEG 200, 0.1 M MES pH 6.0