9NOJ

[2,6,11-2] Shifted DNA assembly with an aspect ratio of (2,6,11) base pairs and 2 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000010
Spacegroup name	P 32
Unit cell lengths	68.231, 68.231, 60.083
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.120 - 3.200
R-factor	0.2873
Rwork	0.284
R-free	0.36480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.768
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.090	59.090	3.752
High resolution limit [Å]	3.200	9.378	3.201
Number of reflections	2406	201	200
<I/σ(I)>	7.4
Completeness [%]	76.9
Redundancy	8.7
CC(1/2)	0.994	1.000	0.679

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate