9NOG

[0,6,13-2] Shifted DNA assembly with an aspect ratio of (0,6,13) base pairs and 2 nt sticky ends containing a semi-junction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920105
Spacegroup name	P 41
Unit cell lengths	69.630, 69.630, 82.325
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.580 - 4.890
R-factor	0.1587
Rwork	0.155
R-free	0.24090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.782
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	69.630	69.630	5.747
High resolution limit [Å]	4.890	10.298	4.890
Number of reflections	1255	209	210
<I/σ(I)>	5.3
Completeness [%]	86.1
Redundancy	6.4
CC(1/2)	0.999	0.999	0.818

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate