9NOF

[7,7,6-1] Shifted tensegrity triangle with an (arm,center,arm) distribution of (7,7,6) base pairs and 1 nt sticky ends, with hexagonal symmetry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.991870
Spacegroup name	H 3
Unit cell lengths	106.503, 106.503, 90.999
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.550 - 4.080
R-factor	0.1856
Rwork	0.183
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.844
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	64.778	64.778	4.504
High resolution limit [Å]	4.076	10.019	4.076
Number of reflections	2083	206	208
<I/σ(I)>	6.8
Completeness [%]	87.3
Redundancy	10.6
CC(1/2)	0.998	0.997	0.095

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate