9NO3
The bifunctional arabinofuranosidase/xylosidase from metagenome of Pseudacanthotermes militaris.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS SIRIUS BEAMLINE MANACA |
| Synchrotron site | LNLS SIRIUS |
| Beamline | MANACA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-10 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.977180 |
| Spacegroup name | P 1 |
| Unit cell lengths | 76.369, 85.774, 90.601 |
| Unit cell angles | 77.34, 68.05, 63.76 |
Refinement procedure
| Resolution | 44.940 - 2.050 |
| R-factor | 0.1806 |
| Rwork | 0.178 |
| R-free | 0.23850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.932 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.940 | 1.960 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.407 | 2.009 |
| Rmeas | 0.487 | 2.449 |
| Rpim | 0.263 | 1.375 |
| Total number of observations | 469926 | 20520 |
| Number of reflections | 140198 | 6662 |
| <I/σ(I)> | 2.5 | 0.8 |
| Completeness [%] | 96.9 | |
| Redundancy | 3.4 | 3.1 |
| CC(1/2) | 0.929 | 0.202 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 41 % w/v PEG 4,000, 150 mM Tris buffer pH 8.0, and 30 mM calcium chloride |
