9NNX

[7,8,4-2] Shifted tensegrity triangle with an (arm,center,arm) distribution of (7,8,4) base pairs and 2 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-13
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.000000
Spacegroup name	P 63
Unit cell lengths	131.388, 131.388, 50.850
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.210 - 5.430
R-factor	0.1264
Rwork	0.124
R-free	0.14420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.894
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.694	65.694	6.291
High resolution limit [Å]	5.430	11.316	5.430
Number of reflections	1275	212	210
<I/σ(I)>	16.3
Completeness [%]	86.3
Redundancy	18.9
CC(1/2)	0.999	0.999	0.677

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate