9NNQ

GNAT family acetyltransferase EryM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	P 21 3
Unit cell lengths	152.846, 152.846, 152.846
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.080 - 2.500
Rwork	0.179
R-free	0.21710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.049
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.080	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.140	1.063
Number of reflections	41204	4044
<I/σ(I)>	8.63	1.56
Completeness [%]	99.8	99.17
Redundancy	10.2	9.9
CC(1/2)	0.995	0.503

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277.15	1.8 M (NH4)2SO4, 3% (w/v) 2- methyl-2,4-pentanediol, 0.2 M NaCl, 0.1 M sodium cacodylate pH 6