9NMM

[4,7,9-1] Shifted tensegrity triangle with an (arm,center,arm) distribution of (4,7,9) base pairs and 1 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.991870
Spacegroup name	H 3
Unit cell lengths	108.163, 108.163, 86.308
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.760 - 4.860
R-factor	0.1372
Rwork	0.135
R-free	0.17220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.886
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.473	63.473	5.665
High resolution limit [Å]	4.857	10.124	4.857
Number of reflections	1176	195	198
<I/σ(I)>	6.5
Completeness [%]	84.4
Redundancy	9.5
CC(1/2)	0.999	0.999	0.429

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate