9NM6

[3,8,9-1] Shifted tensegrity triangle with an (arm,center,arm) distribution of (3,8,9) base pairs and 1 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.991870
Spacegroup name	P 63
Unit cell lengths	134.363, 134.363, 47.695
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.980 - 6.580
R-factor	0.23
Rwork	0.228
R-free	0.25170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.801
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.182	67.182	7.697
High resolution limit [Å]	6.578	11.325	6.578
Number of reflections	848	212	212
<I/σ(I)>	7.9
Completeness [%]	90.2
Redundancy	19.2
CC(1/2)	0.985	0.985	0.137

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate