9NM3

Crystal structure of FBF-1 RBD+CT complexed with compact FBE RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 2 21
Unit cell lengths	42.832, 43.600, 253.278
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.230 - 2.650
R-factor	0.2184
Rwork	0.213
R-free	0.26410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.438
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.640	2.640
Rmerge	0.203	1.323
Number of reflections	13803	607
<I/σ(I)>	8.9
Completeness [%]	93.9
Redundancy	6.3
CC(1/2)	0.956	0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEG 8000, 0.2 M NaCl, 0.1 M CAPS, pH 10.5