9NLR
Crystal structure of human glutamine synthetase in complex with ADP and phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.085, 213.501, 199.942 |
| Unit cell angles | 90.00, 96.26, 90.00 |
Refinement procedure
| Resolution | 45.810 - 2.300 |
| R-factor | 0.1824 |
| Rwork | 0.181 |
| R-free | 0.21510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.560 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_5484: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.490 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.134 | 0.896 |
| Rmeas | 0.159 | 1.057 |
| Rpim | 0.085 | 0.557 |
| Total number of observations | 1156441 | 58786 |
| Number of reflections | 338227 | 16673 |
| <I/σ(I)> | 9.1 | 1.7 |
| Completeness [%] | 99.8 | |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.992 | 0.604 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 291 | 30% (v/v) 2-methyl-2,4-pentanediol, 100 mM Tris pH 8.5, 500 mM NaCl, 8% (w/v) PEG 8000 |
