9NLN
Crystal Structure of Coxakievirus B3 IRES Domain V in Complex with a Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-01 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.927, 84.767, 109.671 |
| Unit cell angles | 90.00, 106.72, 90.00 |
Refinement procedure
| Resolution | 33.530 - 3.000 |
| R-factor | 0.2179 |
| Rwork | 0.216 |
| R-free | 0.26360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.483 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX (1.20.1_4487) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.533 | 3.047 |
| High resolution limit [Å] | 2.996 | 2.996 |
| Number of reflections | 37609 | 1928 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 97.0 | 99.3 |
| Redundancy | 3 | 3.1 |
| CC(1/2) | 0.998 | 0.392 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | PEG 3350, MES pH 6.5, Calcium chloride, Glycerol |
