9NLM
GloR with selenomethionine labels
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97961, 0.97944, 0.91161 |
| Spacegroup name | P 61 |
| Unit cell lengths | 149.078, 149.078, 75.813 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.430 - 2.500 |
| R-factor | 0.2181 |
| Rwork | 0.214 |
| R-free | 0.25210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | unpublished |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.149 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.430 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.078 | 1.239 |
| Rmeas | 0.085 | 1.356 |
| Rpim | 0.033 | 0.540 |
| Number of reflections | 25341 | 1726 |
| <I/σ(I)> | 16 | 2.1 |
| Completeness [%] | 99.5 | 97.6 |
| Redundancy | 12.9 | 11.7 |
| CC(1/2) | 0.999 | 0.827 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 4 % Tacsimate pH 7.0, 12 % PEG3350 |
