9NLL
[0,7,13-->14] Shifted tensegrity triangle with an (arm,center,arm) distribution of (0,7,13) base pairs and 1 nt sticky ends containing a semi-junction that becomes left-handed
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-19 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.991870 |
| Spacegroup name | H 3 |
| Unit cell lengths | 112.826, 112.826, 67.542 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.570 - 6.730 |
| R-factor | 0.1108 |
| Rwork | 0.108 |
| R-free | 0.23160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.708 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 56.413 | 56.413 | 7.823 |
| High resolution limit [Å] | 6.603 | 9.802 | 6.603 |
| Number of reflections | 540 | 178 | 182 |
| <I/σ(I)> | 7 | ||
| Completeness [%] | 90.8 | ||
| Redundancy | 8.2 | ||
| CC(1/2) | 0.996 | 0.995 | 0.185 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM MOPS, 1.25 M magnesium sulfate |
