9NLK

[0,7,13,P-->14] Shifted tensegrity triangle with an (arm,center,arm) distribution of (0,7,13) base pairs, 1 nt sticky ends, and 5' phosphates containing a semi-junction that becomes left-handed

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.991870
Spacegroup name	H 3
Unit cell lengths	115.243, 115.243, 67.158
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.270 - 7.420
R-factor	0.1747
Rwork	0.169
R-free	0.31200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.728
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.621	57.621	9.872
High resolution limit [Å]	7.420	9.937	7.422
Number of reflections	370	185	185
<I/σ(I)>	3.1
Completeness [%]	84.5
Redundancy	8.3
CC(1/2)	0.963	0.961	0.748

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate