9NLI

GloR, native, unmodified

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	99.945, 117.303, 34.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.040 - 2.600
R-factor	0.2406
Rwork	0.234
R-free	0.30040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.050
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.100	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.122	1.132
Rmeas	0.142	1.317
Rpim	0.073	0.668
Number of reflections	13091	1556
<I/σ(I)>	8.2	1.8
Completeness [%]	99.9	100
Redundancy	6.9	7.2
CC(1/2)	0.998	0.855

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	4% Tacsimate pH 8.0, 20% PEG3350