9NLD
O-acetylserine sulfhydrylase A (CysK) from Neisseria gonorrhoeae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.740, 62.180, 105.670 |
| Unit cell angles | 90.00, 90.08, 90.00 |
Refinement procedure
| Resolution | 37.070 - 2.500 |
| R-factor | 0.2004 |
| Rwork | 0.198 |
| R-free | 0.25520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.557 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.530 | 2.580 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.056 | 0.654 |
| Number of reflections | 47132 | 4605 |
| <I/σ(I)> | 19.2 | 2.6 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7 | 7 |
| CC(1/2) | 0.999 | 0.834 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 0.1 M HEPES pH 7.5, 4% polyethylene glycol 400 (v/v), 2.5 M ammonium sulfate |
