9NL8

[3,7,10-1] Shifted tensegrity triangle with an (arm,center,arm) distribution of (3,7,10) base pairs and 1 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.991870
Spacegroup name	H 3
Unit cell lengths	107.966, 107.966, 92.483
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.010 - 3.770
R-factor	0.3039
Rwork	0.299
R-free	0.35060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.826
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.752	65.752	4.121
High resolution limit [Å]	3.768	10.448	3.768
Number of reflections	2729	195	196
<I/σ(I)>	10.1
Completeness [%]	87.0
Redundancy	9.1
CC(1/2)	0.507	0.999	0.439

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate