9NKZ
Crystal structure of Fab MAM01 in complex with NANP6 peptide from circumsporozoite protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-18 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.042, 71.531, 136.776 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.770 - 1.480 |
| R-factor | 0.1913 |
| Rwork | 0.191 |
| R-free | 0.20980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.238 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.510 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.071 | 0.506 |
| Rpim | 0.033 | 0.216 |
| Number of reflections | 77121 | 3808 |
| <I/σ(I)> | 45.1 | |
| Completeness [%] | 98.8 | 99.5 |
| Redundancy | 5.8 | |
| CC(1/2) | 0.990 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.04 M potassium dihydrogen phosphate, 20% (v/v) glycerol and 16% (w/v) polyethylene glycol 8000 |
